Journal:

Article Title: The type I activin receptor ActRIB is required for egg cylinder organization and gastrulation in the mouse

doi:

Figure Lengend Snippet: Analysis of expression of lineage-specific genes in the ActRIB−/− embryos. Hemotoxylin and eosin (H/E) stained sections (A,E) and immunostaining with SSEA-1 (B,F), Troma-1 (C,G) and anti-laminin (D,H) antibodies of the wild-type (A–D) and mutant (E–H) embryo sections at E6.5. The section in A is adjacent to that in B; the section in E is adjacent to that in F. Other sections are from different embryos. In each panel, the ectoplacental cone (or the proximal region) is positioned toward the top. The two clusters of stained epiblast cells in E are marked with arrowheads.

Article Snippet: The primary monoclonal or polyclonal antibodies used are Troma-1 (1:100); SSEA-1 (1:100) (from Developmental Studies Hybridoma Bank), and anti-laminin antibody (1: 200) (Sigma).

Techniques: Expressing, Staining, Immunostaining, Mutagenesis

Journal:

Article Title: T cell-mediated vascular dysfunction of human allografts results from IFN-? dysregulation of NO synthase

doi: 10.1172/JCI200421767

Figure Lengend Snippet: Effects of allogeneic T cells on arterial graft function at 1 week in vivo. Transplanted human arterial segments were recovered from mice injected with saline (open squares) or PBMCs (filled squares) 7–9 days before harvest (n = 5 pairs from four experiments). (A–D) Response curves. Restriction response to various concentrations of PGF2α (A) and relaxation response curves for nitroprusside (B), bradykinin (C), or substance P (D) after preconstriction with PGF2α. *P < 0.05; **P < 0.01; #P < 0.001 vs. saline control. mN, milliNewtons. (E) Immunohistochemistry of graft sections stained for human and murine (inset) CD31, human CD45, and HLA-DR. The staining for human CD45 is indistinguishable from that for human CD3 (data not shown). Original magnification, ×200.

Article Snippet: Immunostaining was performed as described ( 14 ) using the following primary mAb’s: mouse anti–human CD31, mouse anti–human HLA-DR (α-chain), and mouse anti–human CD45 (all from DAKO Corp.); mouse anti–human CD3 (R&D Systems Inc.); mouse anti-iNOS (Transduction Laboratories); and rat anti–mouse CD31 (BD Biosciences).

Techniques: In Vivo, Injection, Saline, Control, Immunohistochemistry, Staining

Journal:

Article Title: T cell-mediated vascular dysfunction of human allografts results from IFN-? dysregulation of NO synthase

doi: 10.1172/JCI200421767

Figure Lengend Snippet: Effects of allogeneic T cells on arterial graft function at 2 weeks in vivo. Transplanted human arterial segments were recovered from mice injected with saline (open squares) or PBMCs (filled squares) for 13–15 days (n = 4–7 pairs from four to six experiments). (A–D) Response curves. Constriction response to various concentrations of PGF2α (A) and relaxation response to bradykinin (B), nitroprusside (C), or YC-1 (D) after preconstriction with PGF2α. *P < 0.05; **P < 0.01; #P < 0.001 vs. saline control. (E) Immunohistochemistry of graft sections stained for human and murine (inset) CD31, human CD3, and iNOS. iNOS expression was highly localized to infiltrating T cells (arrows). Original magnification: CD31 and CD3 panels, ×200; iNOS panels, ×400.

Article Snippet: Immunostaining was performed as described ( 14 ) using the following primary mAb’s: mouse anti–human CD31, mouse anti–human HLA-DR (α-chain), and mouse anti–human CD45 (all from DAKO Corp.); mouse anti–human CD3 (R&D Systems Inc.); mouse anti-iNOS (Transduction Laboratories); and rat anti–mouse CD31 (BD Biosciences).

Techniques: In Vivo, Injection, Saline, Control, Immunohistochemistry, Staining, Expressing

Journal:

Article Title: T cell-mediated vascular dysfunction of human allografts results from IFN-? dysregulation of NO synthase

doi: 10.1172/JCI200421767

Figure Lengend Snippet: Effects of IFN-γ neutralization on T cell–mediated graft dysfunction in vivo. Transplanted human arterial segments were recovered from mice injected with saline alone (open squares) or PBMCs together with anti–IFN-γ mAb (filled triangles) or control mAb (IgG2a) (open triangles) for 2 weeks (n = 5 matched triplicates from three experiments). (A and B) Endothelium-independent responses to various concentrations of PGF2α (A) and nitroprusside (B). (C and D) Endothelium-dependent response to bradykinin before (C) and after (D) treatment with L-NAME. *P < 0.05; **P < 0.01; #P < 0.001 vs. PBMC + IgG2a group, n = 3–5. (E) Immunohistochemistry of graft sections stained for human CD3 and HLA-DR. The luminal HLA-DR–positive cells are also human CD31–positive (data not shown). Goat anti-mouse secondary Ab alone did not stain the grafts. Original magnification: CD3 panels, ×200; HLA-DR panels, ×400. (F and G) RNA transcript levels of iNOS (F) and eNOS (G) in whole tissue sections analyzed by quantitative RT-PCR.

Article Snippet: Immunostaining was performed as described ( 14 ) using the following primary mAb’s: mouse anti–human CD31, mouse anti–human HLA-DR (α-chain), and mouse anti–human CD45 (all from DAKO Corp.); mouse anti–human CD3 (R&D Systems Inc.); mouse anti-iNOS (Transduction Laboratories); and rat anti–mouse CD31 (BD Biosciences).

Techniques: Neutralization, In Vivo, Injection, Saline, Control, Immunohistochemistry, Staining, Quantitative RT-PCR

Journal:

Article Title: T cell-mediated vascular dysfunction of human allografts results from IFN-? dysregulation of NO synthase

doi: 10.1172/JCI200421767

Figure Lengend Snippet: Effects of TNF neutralization on T cell–mediated graft dysfunction in vivo. Transplanted human arterial segments were recovered from mice injected with saline alone (open squares) or PBMCs together with anti-TNF mAb (filled triangles) or control mAb (IgG1) (open triangles) for 2 weeks (n = 4 matched triplicates from two experiments). (A–C) Concentration-dependent responses to PGF2α (A), nitroprusside (B), and bradykinin (C). *P < 0.05; **P < 0.01; #P < 0.001 vs. PBMC + IgG1 group; n = 3–4. (D) Immunohistochemistry of graft sections stained for human CD3 and HLA-DR. Original magnification: CD3 panels, ×200; HLA-DR panels, ×400. (E and F) RNA transcript levels of iNOS (E) and eNOS (F) in whole tissue sections analyzed by quantitative RT-PCR.

Article Snippet: Immunostaining was performed as described ( 14 ) using the following primary mAb’s: mouse anti–human CD31, mouse anti–human HLA-DR (α-chain), and mouse anti–human CD45 (all from DAKO Corp.); mouse anti–human CD3 (R&D Systems Inc.); mouse anti-iNOS (Transduction Laboratories); and rat anti–mouse CD31 (BD Biosciences).

Techniques: Neutralization, In Vivo, Injection, Saline, Control, Concentration Assay, Immunohistochemistry, Staining, Quantitative RT-PCR